Lily cultivars are also classified into 8 groups (Oriental hybrid, LA-hybrid, OT-hybrid, Asiatic hybrid, LO-hybrid, Longiflorum, and Aurelian & Trumpet), which are cultivated to create ornamental value.
Today, more than 10,000 cultivars have been registered in the Royal Horticulture Society, showcasing great value around the world. Based on their morphological classification, these species are divided into the following 8 sections: Section Martagon, Sect. Lilies have originated from the Himalayas and the southwest of China, where there are currently about 51 species/varieties present. Lilies are endemic to the cold and temperate regions of the northern hemisphere. Lilium is a genus of flowering plants which includes 110-115 species of lily. The method can be used in the classification of wild Lilium species, especially endangered species, and also provides an effective method for selective lily breeding. ConclusionsĪ rapid and reliable DNA barcoding method was developed for all 45 wild Lilium species by using ITS, trnP-psaJ-rpI33, and psbB-psbH as DNA barcoding markers.
We showed that a set of three novel DNA barcodes ( ITS + trnP-psaJ-rpI33 + psbB-psbH) could be efficiently used as a genetic marker to distinguish between lily species, as assessed by methods including DNAsp, BI and ML tree, and Pair Wise Group (PWG).
In this study, we assessed five commonly used DNA barcode candidates ( ITS, rbc L, ycf1b, mat K and psbA-trnH) and five novel barcode candidates obtained from highly variable chloroplast genomic regions ( trnL-trnF, trnS-trnG, trnF-ndhJ, trnP-psaJ-rpI33 and psbB-psbH). To develop a simple and reliable identification system for Lilium, 45 representative species from 6 sections were used to develop a DNA barcoding system, which was based on DNA sequence polymorphisms. To date, no efficient species-specific markers have been developed for classifying wild lily species, which poses an issue with further characterizing its medicinal properties. Species within the Lilium genus share very similar morphology and macroscopic characteristics, thus they cannot be easily and clearly distinguished from one another. Lilium is an important ornamental bulb, possesses medicinal properties, and is also edible.